aicar catalog 99447 33 3 tocris bioscience (Tocris)

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Tocris aicar catalog 99447 33 3 tocris bioscience

<t>AICAR-mediated</t> AMPK activation rescues DEDT-induced retinal damage. ( A ) The effect of the AMPK activator AICAR on retinal cell viability under high glucose conditions with DEDT exposure. ( B ) Quantitative PCR was performed to analyze the mRNA expression of key components in the AMPK/Nrf2/HO-1 signaling pathway. ( C-D ) Protein levels of AMPK, Nrf2, and HO-1 were quantified by WB and quantitative analysis. ( E ) The expression of the anti-apoptotic protein BCL-2 was evaluated using ELISA. ( F-G ) Levels of tight junction proteins. ( H ) Inflammatory cytokines, including IL-6 and IL-8, were quantified by ELISA. All DEDT treatments were conducted under high-glucose exposure. Data are presented as mean ± SD ( n = 6). Statistical significance was determined by one-way ANOVA followed by Tukey’s post-hoc test, **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns = not significant. AICAR: AMPK activator.

Aicar Catalog 99447 33 3 Tocris Bioscience, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aicar+catalog+99447+33+3+tocris+bioscience/AICAR/pmc12992771-23-11-14
Average 95 stars, based on 135 article reviews

aicar catalog 99447 33 3 tocris bioscience - by Bioz Stars, 2026-11

95/100 stars

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1) Product Images from "Organophosphate pesticide DEDT promotes diabetic retinopathy progression via AMPK/Nrf2/HO-1 pathway"

Article Title: Organophosphate pesticide DEDT promotes diabetic retinopathy progression via AMPK/Nrf2/HO-1 pathway

Journal: Scientific Reports

doi: 10.1038/s41598-026-37183-w

AICAR-mediated AMPK activation rescues DEDT-induced retinal damage. ( A ) The effect of the AMPK activator AICAR on retinal cell viability under high glucose conditions with DEDT exposure. ( B ) Quantitative PCR was performed to analyze the mRNA expression of key components in the AMPK/Nrf2/HO-1 signaling pathway. ( C-D ) Protein levels of AMPK, Nrf2, and HO-1 were quantified by WB and quantitative analysis. ( E ) The expression of the anti-apoptotic protein BCL-2 was evaluated using ELISA. ( F-G ) Levels of tight junction proteins. ( H ) Inflammatory cytokines, including IL-6 and IL-8, were quantified by ELISA. All DEDT treatments were conducted under high-glucose exposure. Data are presented as mean ± SD ( n = 6). Statistical significance was determined by one-way ANOVA followed by Tukey’s post-hoc test, **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns = not significant. AICAR: AMPK activator.

Figure Legend Snippet: AICAR-mediated AMPK activation rescues DEDT-induced retinal damage. ( A ) The effect of the AMPK activator AICAR on retinal cell viability under high glucose conditions with DEDT exposure. ( B ) Quantitative PCR was performed to analyze the mRNA expression of key components in the AMPK/Nrf2/HO-1 signaling pathway. ( C-D ) Protein levels of AMPK, Nrf2, and HO-1 were quantified by WB and quantitative analysis. ( E ) The expression of the anti-apoptotic protein BCL-2 was evaluated using ELISA. ( F-G ) Levels of tight junction proteins. ( H ) Inflammatory cytokines, including IL-6 and IL-8, were quantified by ELISA. All DEDT treatments were conducted under high-glucose exposure. Data are presented as mean ± SD ( n = 6). Statistical significance was determined by one-way ANOVA followed by Tukey’s post-hoc test, **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns = not significant. AICAR: AMPK activator.

Techniques Used: Activation Assay, Real-time Polymerase Chain Reaction, Expressing, Enzyme-linked Immunosorbent Assay

CCK-8 Assay:

Article Title: Organophosphate pesticide DEDT promotes diabetic retinopathy progression via AMPK/Nrf2/HO-1 pathway
Article Snippet: .. Streptozotocin (STZ), Catalog: S0130, Sigma-Aldrich; Diethyl Dithiophosphate (DEDT), Catalog: 24617-47-0, ChemService; AICAR, Catalog: 99447-33-3, Tocris Bioscience; Cell Counting Kit-8 (CCK-8), Catalog: C0037, Beyotime Biotechnology; TRIzol Reagent, Catalog: 15,596,026, Thermo Fisher Scientific; SYBR Green Master Mix, Catalog: 4,309,155, Applied Biosystems; Cell Counting Kit-8 (CCK-8), Catalog: C0037, Beyotime Biotechnology; ZO-1 ELISA Kit, Catalog: PT518, Beyotime Biotechnology; Occludin ELISA Kit, Catalog: PT519, Beyotime Biotechnology; Claudin-5 ELISA Kit, Catalog: PT521, Beyotime Biotechnology; IL-6 ELISA Kit, Catalog: PI330, Beyotime Biotechnology; TNF-α ELISA Kit, Catalog: PI305, Beyotime Biotechnology;; IL-8 ELISA Kit, Catalog: PI331, Beyotime Biotechnology;; IL-1β ELISA Kit, Catalog: PI303, Beyotime Biotechnology; Bax ELISA Kit, Catalog: PA102, Beyotime Biotechnology; BCL-2 ELISA Kit, Catalog: PA101, Beyotime Biotechnology; Phospho-AMPK (Thr172) ELISA Kit, Catalog: PT428, Beyotime Biotechnology; Nrf2 ELISA Kit, Catalog: PT588, Beyotime Biotechnology; HO-1 ELISA Kit, Catalog: PT592, Beyotime Biotechnology; Glutathione (GSH) Assay Kit, Catalog: S0053, Beyotime Biotechnology; Malondialdehyde (MDA) test kit, Catalog: S0131S, Beyotime Biotechnology; Superoxide dismutase (SOD) test kit, Catalog: S0101, Beyotime Biotechnology; Catalase (CAT) test kit, Catalog: S0051, Beyotime Biotechnology. .. Sprague-Dawley rats (8–10 weeks old, 250–300 g) were obtained from the Animal Experiment Center of Anhui Medical University and were maintained under appropriate conditions.

Article Title: Organophosphate pesticide DEDT promotes diabetic retinopathy progression via AMPK/Nrf2/HO-1 pathway.
Article Snippet: .. Streptozotocin (STZ), Catalog: S0130, Sigma-Aldrich; Diethyl Dithiophosphate (DEDT), Catalog: 24617- 47-0, ChemService; AICAR, Catalog: 99447-33-3, Tocris Bioscience; Cell Counting Kit-8 (CCK-8), Catalog: C0037, Beyotime Biotechnology; TRIzol Reagent, Catalog: 15,596,026, Thermo Fisher Scientific; SYBR Green Master Mix, Catalog: 4,309,155, Applied Biosystems; Cell Counting Kit-8 (CCK-8), Catalog: C0037, Beyotime Biotechnology; ZO-1 ELISA Kit, Catalog: PT518, Beyotime Biotechnology; Occludin ELISA Kit, Catalog: PT519, Beyotime Biotechnology; Claudin-5 ELISA Kit, Catalog: PT521, Beyotime Biotechnology; IL-6 ELISA Kit, Catalog: PI330, Beyotime Biotechnology; TNF-α ELISA Kit, Catalog: PI305, Beyotime Biotechnology;; IL-8 ELISA Kit, Catalog: PI331, Beyotime Biotechnology;; IL-1β ELISA Kit, Catalog: PI303, Beyotime Biotechnology; Bax ELISA Kit, Catalog: PA102, Beyotime Biotechnology; BCL-2 ELISA Kit, Catalog: PA101, Beyotime Biotechnology; Phospho-AMPK (Thr172) ELISA Kit, Catalog: PT428, Beyotime Biotechnology; Nrf2 ELISA Kit, Catalog: PT588, Beyotime Biotechnology; HO-1 ELISA Kit, Catalog: PT592, Beyotime Biotechnology; Glutathione (GSH) Assay Kit, Catalog: S0053, Beyotime Biotechnology; Malondialdehyde (MDA) test kit, Catalog: S0131S, Beyotime Biotechnology; Superoxide dismutase (SOD) test kit, Catalog: S0101, Beyotime Biotechnology; Catalase (CAT) test kit, Catalog: S0051, Beyotime Biotechnology. .. Sprague-Dawley rats (8–10 weeks old, 250–300 g) were obtained from the Animal Experiment Center of Anhui Medical University and were maintained under appropriate conditions.

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